TFS Talos Arctica G2

A) Operator (Dr. Julio Ortiz) captured loading sample on Talos Arctica 200 kV B). Trainer and user (Dr. Saba Shahzad and Esther Hudina) screening the samples on Talos Arctica 200kV C). Micrograph of vitrified tobacco mosaic virus (TMV) as viewed at 100 kX nominal magnification in Talos Arctica 200 kV (picture credit: Dr. Olivera Korculanin)

Typical applications

The Talos Arctica (ThermoFisher Scientific) is a 200 kV microscope suitable for cryo-sample screening and high-resolution data acquisition. Main applications are single-particle imaging and cryo-tomography acquisitions. The Talos Arctica is equipped with a K3 Bioquantum energy filter which has power to resolve standard Apoferritin samples down to 2Å and ~200 acquisitions/hour is the current rate of collection in counting mode.

Technical specifications

Operating voltage	200 kV
TEM magnification range	Up to130,000X with the K3 detector (0.65 Å/pixel and 0.3 Å/pixel in super resolution mode)
Ion source	X-FEG (High-brightness field emission gun)
Operating conditions	Cryo / liquid nitrogen conditions
Detector	Ceta 4kx4k 16M Camera with Peltier cooled CMOS sensor Gatan K3 direct electron detector with BioQuantum energy filter
Configuration	TFS Autoloader for 12 grids Automated data acquisition at cryo conditions Volta phase plate
Stage	Cryo-stage with dual tilt holder Computerized 4-axes specimen stage with ±70° alpha tilt
Software	EPU, TIA, SerialEM