Nanometer resolution information of molecular machines, organelles, cells and tissues can also be achieved by collecting a tilt-series of the vitrified sample, which can be computationally reconstructed to a three-dimensional tomogram using the technique of cryo-electron tomography.
The vitrified sample can either be shipped to the ER-C or be vitrified on-site using one of our sample preparation instruments like VitrobotTM, a Manual or the LeicaTM Plunge freezer. Vitrified samples can be further thinned for cryo-EM using our FIB-SEM. We offer sample visualizations by tomography on state-of-the-art cryo-electron microscopes.
Tomograpahy on Talos Arctica (200 kV)
Cells from retinal pigment epithelium (RPE1) viewed at 24 kx nominal magnification with a total dose of 100 e-/ Å2 with 2 e-/ Å2 per tilt (-40 to +40 tilt angle) collected with dose-symmeteric scheme on Talos Arctica 200 kV equipped with a Gatan Bioqunatum K3 Detector.
Data source: Dr. Sabrina Berkamp and Dr. Julio Ortiz, Ernst-Ruska Centre 3 EM facility, Forschungszentrum Jülich, Germany