Tomography acquisition

Nanometer resolution information of molecular machines, organelles, cells and tissues can also be achieved by collecting a tilt-series of the vitrified sample, which can be computationally reconstructed to a three-dimensional tomogram using the technique of cryo-electron tomography.

The vitrified sample can either be shipped to the ER-C or be vitrified on-site using one of our sample preparation instruments like VitrobotTM, a Manual or the LeicaTM Plunge freezer. Vitrified samples can be further thinned for cryo-EM using our FIB-SEM. We offer sample visualizations by tomography on state-of-the-art cryo-electron microscopes