Statistical density thresholding of cryo-EM maps using “false discovery rate” control

Confidence maps are complementary maps generated from cryo-EM maps by means of statistical hypothesis testing and subsequent FDR control. They allow thresholding of EM maps based on the expected amount of background noise visible at the respective threshold and thus allow rigorous error assessment of visible features in the density.

Additional post-processing like local filtering or local amplitude scaling (LocScale) can be incorporated in the framework in order to increase the statistical power.


More information can be found here:

Beckers, M., Jakobi, A. J. & Sachse, C.

Thresholding of cryo-EM density maps by false discovery rate control

(2019). IUCrJ 6, 18-33.