Cryo-EM applications

The Ernst Ruska-Centre cryo-EM user facility is offering technical and scientific support to our academic and industrial users in EM methodologies. We provide access to the complete pipeline from sample preparation to image acquisition on state-of-the-art cryo-electron microscopes. Experienced users can operate the devices with minimal supervision and we will also teach and train new users to be independent. Some of the typical application cases are listed below. Reach out to us to learn more about our booking process and how we can assist you in your research endeavors.


Henderikx, R. J. M., Mann, D., Domanska, A., Dong, J., Shahzad, S., Lak, B., Filopoulou, A., Ludig, D., Grininger, M., Momoh, J., Laanto, E., Oksanen, H. M., Bisikalo, K., Williams, P. A., Butcher, S. J., Peters, P. J. & Beulen, B. W. A. M. M. VitroJet: new features and case studies Acta Cryst. D80 (2024). .

Berkamp S, Daviran D, Smeets M, Caignard A, Jani RA, Sundermeyer P, Jonker C, Gerlach S, Hoffmann B, Lau K, Sachse C. Correlative Light and Electron Cryo-Microscopy Workflow Combining Micropatterning, Ice Shield, and an In-Chamber Fluorescence Light Microscope. Bio Protoc. 2023 Dec 20;13(24):e4901.

Zielinski, M., Peralta Reyes, F.S., Gremer, L. et al. Cryo-EM of Aβ fibrils from mouse models find tg-APPArcSwe fibrils resemble those found in patients with sporadic Alzheimer’s disease. Nat Neurosci 26, 2073–2080 (2023).

Mann, D., Fromm, S.A., Martinez-Sanchez, A. et al. Atg18 oligomer organization in assembled tubes and on lipid membrane scaffolds. Nat Commun 14, 8086 (2023).

de Witt, J., Molitor, R., Gätgens, J., Ortmann de Percin Northumberland, C., Kruse, L., Polen, T. et al.  Biodegradation of poly(ester-urethane) coatings by Halopseudomonas formosensisMicrobial Biotechnology, 17, e14362. (2024).

Bitzenhofer, N.L., Höfel, C., Thies, S., Weiler, A.J., Eberlein, C., Heipieper, H.J. et al.  Exploring engineered vesiculation by Pseudomonas putida KT2440 for natural product biosynthesis. Microbial Biotechnology, 17, e14312. (2024).

Junglas B, Huber ST, Heidler T, Schlösser L, Mann D, Hennig R, Clarke M, Hellmann N, Schneider D, Sachse C. PspA adopts an ESCRT-III-like fold and remodels bacterial membranes. Cell. 2021 Jul 8;184(14):3674-3688.e18.

Huber ST, Mostafavi S, Mortensen SA, Sachse C. Structure and assembly of ESCRT-III helical Vps24 filaments. Sci Adv. 2020 Aug 19;6(34):eaba4897.

Jakobi AJ, Huber ST, Mortensen SA, Schultz SW, Palara A, Kuhm T, Shrestha BK, Lamark T, Hagen WJH, Wilmanns M, Johansen T, Brech A, Sachse C. Structural basis of p62/SQSTM1 helical filaments and their role in cellular cargo uptake. Nat Commun. 2020 Jan 23;11(1):440.