SPRING (S ingle P article R econstruction from I mages of kN own G eometry) is a single-particle based helical reconstruction package for electron cryo-micrographs and has been used to determine 3D structures of a variety of highly ordered and less ordered specimens. Spring provides the entire single-particle based work-flow required for helical reconstruction including:
– Classification
– Helical symmetry determination and refinement tools
– High-resolution structure refinement
– Multi-symmetry structure refinement
Spring is still maintained in the Sachse lab at the Ernst-Ruska Centre of the Research Centre Juelich for the Unix operating systems of MacOSX and Linux.
Ambroise Desfosses, Rodolfo Ciuffa, Irina Gutsche, Carsten Sachse,
SPRING – An image processing package for single-particle based helical reconstruction from electron cryomicrographs,
Journal of Structural Biology, Volume 185, Issue 1, 2014, Pages 15-26, ISSN 1047-8477,
https://doi.org/10.1016/j.jsb.2013.11.003.