Quantitative 3D-volumetric analysis of e.g. synaptic structures to obtain information about the geometry under normal or healthy and (genetically or pathologically) altered conditions.
The methods are established for human and animal brain slices, but can be adapted to other tissues or cell culture preparations and can be combined for example with correlative light- and electron microscopy or FIB-SEM (Ali et al. Glia 2020; Rollenhagen et al. Int J Mol Sci 2020).
