FEI Titan G2 60-300 HOLO


The FEI Titan G2 60-300 HOLO is a unique fourth generation transmission electron microscope, which has been specifically designed for the investigation of electromagnetic fields of materials using off-axis electron holography. It has a Lorentz lens to allow magnetic field free imaging plus two electron biprisms, which in combination enable more uniform holographic fringes to be used. The instrument also has an ultra-wide objective lens pole piece gap which is ideal for in situ experiments. For these purposes, the FEI Titan G2 60-300 HOLO is equipped with a Schottky type high-brightness electron gun (FEI X-FEG), an image Cs corrector (CEOS), a post-column energy filter system (Gatan Tridiem 865 ER) as well as a 4 megapixel CCD system (Gatan UltraScan 1000 XP). Typical examples of use and technical specifications for the instrument are given below.

Typical Applications and Limitations of Use

The configuration of the FEI Titan G2 60-300 HOLO allows a variety of advanced transmission electron microscopy techniques to be applied to a wide variety of solid-state materials. These techniques include high-resolution transmission electron microscopy (HRTEM), electron energy-loss spectroscopy (EELS), energy filtered transmission electron microscopy (EFTEM), scanning transmission electron microscopy (HRSTEM) with high-angle annular dark field (HAADF) STEM imaging, off-axis electron holography (EH), Lorentz microscopy, electron tomography (ET) and combinations of these techniques.

Investigation of aqueous, contaminated, ferromagnetic or organic samples with the FEI Titan G2 60-300 HOLO is possible after discussion with both of the instruments officers.

Sample Environment

Using dedicated cooling or heating stages, the FEI Titan G2 60-300 HOLO will allow samples to be investigated either at room temperature or under liquid nitrogen cooling conditions at a vacuum level of about 10–8 mbar. Besides this standard setup, the sample environment can be adapted to various conditions, e.g. the thermal treatment or the application of external electric or magnetic fields to samples, making use of a wide portfolio of in situ TEM holders available through the ER-C user services.

Technical Specifications

Electron acceleration voltage60 kV … 300 kV
Electron sourceSchottky X-FEG
Information limit (TEM) @ 300 kV< 120 pm
Resolution (STEM) @ 300 kV< 180 pm
C-TWIN objective lens11 mm
Objective lens Cs (Lorentz mode)< 100 mm

Specimen Stages

Double tilt low background holder± 70°
Single tilt holder± 70°
Dual-axis tomography holder± 70°, 360º
On axis rotation tomography holder360°
Liquid He holder10 – 60 K
Further in situ specimen stages available 


Peltier cooled Ultrascan 1000 XP charge coupled device camera (CCD) with a readout speed of 4 M pixel sec-1 and a format of 4-megapixel of 14 microns in size. (Gatan)

Tridiem 865 ER image filter (GIF) with fully 2nd and 3rd order and partially 4th order corrected prisms and a maximum field of view of 17 µm for imaging and 120 mR for diffraction analysis and with a 4-megapixel CCD. (Gatan)

Model 3000 HAADF detector. (Fischione)

Instrument Officer

Instrument related Publications

V Migunov, H Ryll, X Zhuge, M Simson, L Strüder, KJ Batenburg, L Houben, and RE Dunin-Borkowski: Rapid low dose electron tomography using a direct electron detection camera, Scientific Reports 5 (2015) 14516.

C Ozsoy-Keskinbora, CB Boothroyd, RE Dunin-Borkowski, PA van Aken and CT Koch: High-resolution and high phase sensitivity low-dose electron holography, Scientific Reports 4 (2014) 7020.

SLY Chang, C Dwyer, CB Boothroyd and RE Dunin-Borkowski: Optimising electron holography in the presence of partial coherence and instabilities, Ultramicroscopy 151 (2015) 37.

C Dwyer, CB Boothroyd, SLY Chang and RE Dunin-Borkowski: Three-wave electron vortex lattices for measuring nanofields, Ultramicroscopy 148 (2015) 25.