{"id":7171,"date":"2023-07-20T14:20:15","date_gmt":"2023-07-20T14:20:15","guid":{"rendered":"https:\/\/er-c.org\/?page_id=7171"},"modified":"2023-08-10T09:53:37","modified_gmt":"2023-08-10T09:53:37","slug":"sample-preparation","status":"publish","type":"page","link":"https:\/\/er-c.org\/index.php\/facilities-2\/facilities-material-science\/sample-preparation\/","title":{"rendered":"Sample Preparation"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large is-resized ticss-68badfa0\"><img decoding=\"async\" src=\"https:\/\/er-c.org\/wp-content\/uploads\/2023\/07\/Sample-Preparation-1024x64.png\" alt=\"\" class=\"wp-image-7204\" style=\"width:1200px\" width=\"1200\" srcset=\"https:\/\/er-c.org\/wp-content\/uploads\/2023\/07\/Sample-Preparation-1024x64.png 1024w, https:\/\/er-c.org\/wp-content\/uploads\/2023\/07\/Sample-Preparation-300x19.png 300w, https:\/\/er-c.org\/wp-content\/uploads\/2023\/07\/Sample-Preparation-768x48.png 768w, https:\/\/er-c.org\/wp-content\/uploads\/2023\/07\/Sample-Preparation-1536x96.png 1536w, https:\/\/er-c.org\/wp-content\/uploads\/2023\/07\/Sample-Preparation-2048x128.png 2048w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n<div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-headline so-widget-sow-headline-default-e68cdc7fd8ad\"\n\t\t\t\n\t\t><div class=\"sow-headline-container \">\n\t\t\t\t\t\t\t<h1 class=\"sow-headline\">\n\t\t\t\t\t\tSample Preparation\t\t\t\t\t\t<\/h1>\n\t\t\t\t\t\t<\/div>\n<\/div>\n\n\n<hr>\n<p style=\"text-align: center;\">Specimen preparation for the transmission electron microscopy is an unavoidable and critical step for any TEM experiments. It is frequently a very challenging task, which may decide about the success or fail of arduously planned TEM experiment. All of the existing methods for the TEM-specimen preparation follow one common principle \u2013 the thinner, the better, which means that the thickness of the TEM-specimen ideally should not exceed 100 nm (10nm for HRTEM). Furthermore, a high quality ultra-thin TEM-specimen should not only reflect the material properties on the nm scale, but also have a homogeneous and clean surface to avoid the measurement artefacts.<\/p>\n\n\n\n<hr \/>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div id=\"pl-gb7171-69da5aa391aea\"  class=\"panel-layout\" ><div id=\"pg-gb7171-69da5aa391aea-0\"  class=\"panel-grid panel-has-style\" ><div class=\"panel-row-style panel-row-style-for-gb7171-69da5aa391aea-0\" ><div id=\"pgc-gb7171-69da5aa391aea-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-gb7171-69da5aa391aea-0-0-0\" class=\"so-panel widget widget_academia-widget-featured-pages widget-promoted-pages panel-first-child panel-last-child widgetopts-SO\" data-index=\"0\" >\n\t\t\t<div class=\"custom-widget-featured-pages\">\n\t\t\t\n\t\t\t\t<h3 class=\"widget-title\">Conventional Sample Preparation<\/h3>\n\t\t\t\t<ul class=\"site-columns-1 site-columns-widget\">\n\t\t\t\t\n\t\t\t\t<li class=\"site-column-widget site-archive-post\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"entry-thumbnail\"><a href=\"https:\/\/er-c.org\/index.php\/facilities-2\/facilities-material-science\/sample-preparation\/sample-preparation-conventional\/\" rel=\"bookmark\"><img loading=\"lazy\" decoding=\"async\" width=\"580\" height=\"380\" src=\"https:\/\/er-c.org\/wp-content\/uploads\/2023\/07\/Sample-Preparation-Conventional-43-580x380.png\" class=\"attachment-thumb-featured-page size-thumb-featured-page wp-post-image\" alt=\"\" \/><\/a><\/div><!-- .entry-thumbnail -->\t\t\t\t\t\t\t<div class=\"entry-preview\">\n\t\t\t\t\t\t\t\t<p class=\"entry-excerpt\">The conventional TEM-Specimen preparation begins with cutting the bulk sample into small pieces followed by several steps of half-automated material abrasion, until the specimen reaches the desired thickness. In a final step, argon ion milling helps to obtain the acquired ultra-thin electron transparent area.<\/p><p class=\"entry-actions\"><span class=\"site-readmore-span\"><a href=\"https:\/\/er-c.org\/index.php\/facilities-2\/facilities-material-science\/sample-preparation\/sample-preparation-conventional\/\" title=\"Continue Reading: Sample Preparation Conventional\" class=\"site-readmore-anchor\" rel=\"bookmark\">Read More<\/a><\/span><\/p>\t\t\t\t\t\t\t<\/div><!-- .entry-preview -->\n\t\t\t\t\t\t<\/li><!-- .site-column .site-column-1 .site-column-widget -->\n\t\t\t\t<\/ul><!-- .site-columns-1 .site-columns-widget-->\n\t\t\t\n\t\t\t<\/div><!-- .custom-widget-featured-pages -->\n\t\t\t\n\t\t\t<\/div><\/div><div id=\"pgc-gb7171-69da5aa391aea-0-1\"  class=\"panel-grid-cell\" ><div class=\"panel-cell-style panel-cell-style-for-gb7171-69da5aa391aea-0-1\" ><div id=\"panel-gb7171-69da5aa391aea-0-1-0\" class=\"so-panel widget widget_academia-widget-featured-pages widget-promoted-pages panel-first-child panel-last-child widgetopts-SO\" data-index=\"1\" >\n\t\t\t<div class=\"custom-widget-featured-pages\">\n\t\t\t\n\t\t\t\t<h3 class=\"widget-title\">FIB Sample Preparation<\/h3>\n\t\t\t\t<ul class=\"site-columns-1 site-columns-widget\">\n\t\t\t\t\n\t\t\t\t<li class=\"site-column-widget site-archive-post\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"entry-thumbnail\"><a href=\"https:\/\/er-c.org\/index.php\/facilities-2\/facilities-material-science\/sample-preparation\/sample-preparation-fib\/\" rel=\"bookmark\"><img loading=\"lazy\" decoding=\"async\" width=\"580\" height=\"380\" src=\"https:\/\/er-c.org\/wp-content\/uploads\/2023\/07\/FIB-43-580x380.png\" class=\"attachment-thumb-featured-page size-thumb-featured-page wp-post-image\" alt=\"\" \/><\/a><\/div><!-- .entry-thumbnail -->\t\t\t\t\t\t\t<div class=\"entry-preview\">\n\t\t\t\t\t\t\t\t<p class=\"entry-excerpt\">In contrast to time-consuming conventional TEM-specimen preparation methods, the focused ion beam (FIB) sputtering in a dual beam SEM system allows to produce high-quality specimens for various TEM studies in a (semi-)automated and much quicker way (within several hours). Modern dual beam FIB\/SEMs use precise piezo-controlled manipulators, various gas injection systems to protect the sample surface, energy dispersive X-ray spectroscopy to measure composition and different detectors to image the specimen during the preparation process. In the dual systems, the imaging process results from using\u00a0 scanning electrons (SEM) whereby scanning of focused gallium ions at the sample surface enables local material abrasion.<\/p><p class=\"entry-actions\"><span class=\"site-readmore-span\"><a href=\"https:\/\/er-c.org\/index.php\/facilities-2\/facilities-material-science\/sample-preparation\/sample-preparation-fib\/\" title=\"Continue Reading: Sample Preparation FIB\" class=\"site-readmore-anchor\" rel=\"bookmark\">Read More<\/a><\/span><\/p>\t\t\t\t\t\t\t<\/div><!-- .entry-preview -->\n\t\t\t\t\t\t<\/li><!-- .site-column .site-column-1 .site-column-widget -->\n\t\t\t\t<\/ul><!-- .site-columns-1 .site-columns-widget-->\n\t\t\t\n\t\t\t<\/div><!-- .custom-widget-featured-pages -->\n\t\t\t\n\t\t\t<\/div><\/div><\/div><\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Specimen preparation for the transmission electron microscopy is an unavoidable and critical step for any TEM experiments. It is frequently a very challenging task, which may decide about the success or fail of arduously planned TEM experiment. All of the existing methods for the TEM-specimen preparation follow one common principle \u2013 the thinner, the better, which means that the thickness of the TEM-specimen ideally should not exceed 100 nm (10nm for HRTEM). Furthermore, a high quality ultra-thin TEM-specimen should not only reflect the material properties on the nm scale, but also have a homogeneous and clean surface to avoid the measurement artefacts.<\/p>\n","protected":false},"author":2,"featured_media":7202,"parent":7169,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/pagebuilder.php","meta":{"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"class_list":["post-7171","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/7171","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/comments?post=7171"}],"version-history":[{"count":8,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/7171\/revisions"}],"predecessor-version":[{"id":7338,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/7171\/revisions\/7338"}],"up":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/7169"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media\/7202"}],"wp:attachment":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media?parent=7171"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}