{"id":6943,"date":"2023-05-23T07:43:00","date_gmt":"2023-05-23T07:43:00","guid":{"rendered":"https:\/\/er-c.org\/?page_id=6943"},"modified":"2023-05-23T07:46:35","modified_gmt":"2023-05-23T07:46:35","slug":"ultrastructural-em-quantitative-3d-volumetric-analysis","status":"publish","type":"page","link":"https:\/\/er-c.org\/index.php\/facilities-2\/life-science\/ultrastructural-electron-microscopy-facility\/ultrastructural-em-applications\/ultrastructural-em-quantitative-3d-volumetric-analysis\/","title":{"rendered":"Ultrastructural-EM Quantitative 3D Volumetric analysis"},"content":{"rendered":"\n
\"\"<\/figure>\n\n\n\n
<\/div>\n\n\n
\n\t\t\t\t\t\t\t

\n\t\t\t\t\t\tQuantitative 3D volumetric analysis\t\t\t\t\t\t<\/h1>\n\t\t\t\t\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div>\n\n\n
<\/div>\n\n\n

Quantitative 3D volumetric analysis of e.g. synaptic structures<\/h3>\n
\n\t

Quantitative 3D-volumetric analysis of e.g. synaptic structures to obtain information about the geometry under normal or healthy and (genetically or pathologically) altered conditions.<\/p>\n

The methods are established for human and animal brain slices, but can be adapted to other tissues or cell culture preparations and can be combined for example with correlative light- and electron microscopy or FIB-SEM (Ali et al. Glia 2020; Rollenhagen et al. Int J Mol Sci 2020).<\/p>\n<\/div>\n<\/div><\/div><\/div>

\n
\n\t
\"\"
3D reconstruction of a dendritic segment (blue) with several synaptic boutons (yellow or transparent yellow) terminating either on dendritic spines or the dendritic shaft. In addition, synaptic (green) and dense-core (magenta) vesicles around active zones (red) are shown. Taken from: Rollenhagen et al. Int J Mol Sci. 2020). Inset: Serial digital TEM images with outlined synaptic structures as used for 3D-reconstructions.<\/figcaption><\/figure>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>\n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

The ER-C Ultrastructural EM user facility is offering technical and scientific support to our academic and industrial users in EM methodologies. We provide access to life science research approaches complementing our high-resolution cryo-EM facility.<\/p>\n","protected":false},"author":2,"featured_media":6287,"parent":6281,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/pagebuilder.php","meta":{"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"class_list":["post-6943","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6943","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/comments?post=6943"}],"version-history":[{"count":2,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6943\/revisions"}],"predecessor-version":[{"id":6946,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6943\/revisions\/6946"}],"up":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6281"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media\/6287"}],"wp:attachment":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media?parent=6943"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}