{"id":6746,"date":"2023-05-12T21:28:28","date_gmt":"2023-05-12T21:28:28","guid":{"rendered":"https:\/\/er-c.org\/?page_id=6746"},"modified":"2023-07-05T10:03:00","modified_gmt":"2023-07-05T10:03:00","slug":"single-particle-acquisition","status":"publish","type":"page","link":"https:\/\/er-c.org\/index.php\/facilities-2\/life-science\/cryo-electron-microscopy-facility\/cryo-em-applications\/single-particle-acquisition\/","title":{"rendered":"Single-particle acquisition"},"content":{"rendered":"\n
\"\"<\/figure>\n\n\n
\n\t\t\t\t\t\t\t

\n\t\t\t\t\t\tSingle-particle acquisition\t\t\t\t\t\t<\/h1>\n\t\t\t\t\t\t<\/div>\n<\/div>\n\n\n
\n

We offer high-throughput single-particle data acquisition on state-of-the-art transmission electron microscopes<\/a> with an acquisition rate of ~200 to ~500 movies\/hour, for our Talos Arctica<\/a> <\/i>and Titan Krios<\/a> instruments respectively. The plunge-frozen sample can be shipped to the ER-C or vitrified on-site using one of our sample preparation devices<\/a>. Samples can be visualized initially<\/a> before high through put data collection is performed.<\/p>\n

We have solved structures of as small as 65 kDa globular protein (5 nm) to 20 nm wide fibrils (see images below).<\/p>\n\n\n

\n\t\t\t\n\t\t\n\t\t\t\n\t\t\tContact us\t\t<\/span>\n\t\t\t<\/a>\n\t<\/div>\n<\/div>\n\n\n
\n\n\n\n
<\/div>\n\n\n\t\t\t\t
\n\t\t\t\t\t\t\t
  • \n\t\t\t\t\t
    \n\t\t\t
    \n\t\t\t\t
    \n
    \n\t\t\"\"\n\t<\/div>\n\n<\/div><\/div><\/div>

    Single particle on Talos Arctica (200 kV)<\/h3>\n
    \n\t

    Structure of Human Haemoglobin (65 kDa protein) at 3 \u00c5 resolution, 100 kx nominal magnification (0.8389 \u00c5\/pixel ), with a total dose of 65 e-\/ \u00c52 captured with Talos Arctica 200 kV<\/a> equipped with a Gatan Bioquantum K3 Detector.<\/p>\n

    Data source: Dr. Daniel Mann, <\/b>Ernst-Ruska Centre 3 EM facility, Forschungszentrum J\u00fclich, Germany.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/li>\n\t\t\t\t

  • \n\t\t\t\t\t
    \n\t\t\t
    \n\t\t\t\t
    \n
    \n\t\t\"\"\n\t<\/div>\n\n<\/div><\/div><\/div>

    Single particle on Talos Arctica (200 kV)<\/h3>\n
    \n\t

    Structure of Ribosome, 100 kx nominal magnification (0.816 \u00c5 \/px), with a total dose of 50 e-\/ \u00c52<\/sup> recorded at Talos Arctica 200 kV<\/a> equipped with a Gatan Bioquantum K3 Detector.<\/p>\n

    Data source: Nuno Bustorff, <\/b>Ernst-Ruska Centre 3 EM facility, Forschungszentrum J\u00fclich, Germany.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/li>\n\t\t\t\t

  • \n\t\t\t\t\t
    \n\t\t\t
    \n\t\t\t\t
    \n
    \n\t\t\"\"\n\t<\/div>\n\n<\/div><\/div><\/div>

    Single particle on Talos Arctica (200 kV)<\/h3>\n
    \n\t

    Structure of SynPspA rods at 3.6 \u00c5 resolution, 100 kx nominal magnification (0.8389 \u00c5\/pixel), with a total dose of 50 e-\/ \u00c52<\/sup> recorded at Talos Arctica 200 kV<\/a> equipped with a Gatan Bioqunatum K3 Detector.<\/p>\n

    Data source: Dr. Benedikt Junglas, <\/b>Ernst-Ruska Centre 3 EM facility, Forschungszentrum J\u00fclich, Germany.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/li>\n\t\t\t\t

  • \n\t\t\t\t\t
    \n\t\t\t
    \n\t\t\t\t
    \n
    \n\t\t\"\"\n\t<\/div>\n\n<\/div><\/div><\/div>

    Single particle on Titan Krios (300 kV)<\/h3>\n
    \n\t

    Structure of Tobacco Mosaic Virus, 96 kx nominal magnification (0.808 \u00c5\/pixel ), with a total dose of 40 e-\/ \u00c52<\/sup> recorded on Titan Krios 300 kV<\/a> equipped with a Falcon IV Detector.<\/p>\n

    Data source: <\/b>Dr. Daniel Mann<\/strong>, Ernst-Ruska Centre 3 EM facility, Forschungszentrum J\u00fclich, Germany.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/li>\n\t\t<\/ul>\t\t\t\t

      \n\t\t\t\t\t\t\t\t\t\t\t
    1. <\/a><\/li>\n\t\t\t\t\t\t\t\t\t\t\t
    2. <\/a><\/li>\n\t\t\t\t\t\t\t\t\t\t\t
    3. <\/a><\/li>\n\t\t\t\t\t\t\t\t\t\t\t
    4. <\/a><\/li>\n\t\t\t\t\t\t\t\t\t<\/ol>\n\n\t\t\t\t
      \n\t\t\t\t\t\n\t\t\t\t\t\t<\/em>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\n\t\t\t\t
      \n\t\t\t\t\t\n\t\t\t\t\t\t<\/em>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div><\/div>\n\n\n
      <\/div>\n\n\n\n
      <\/div>\n\n\n
      \n\t\t\t\t\t\t
      \n\t\t\t\t\t\t
      <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"

      We offer high-throughput single-particle data acquisition on state-of-the-art transmission electron microscopes with an acquisition rate of ~200 to ~500 movies\/hour, for our Talos Arctica and Titan Krios instruments respectively. The plunge-frozen sample can be shipped to the ER-C or vitrified on-site using one of our sample preparation devices. Samples can be visualized initially before high through put data collection is performed.
      \nWe have solved structures of as small as 65 kDa globular protein (5 nm) to 20 nm wide fibrils.<\/p>\n","protected":false},"author":2,"featured_media":6780,"parent":6211,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/pagebuilder.php","meta":{"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"class_list":["post-6746","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6746","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/comments?post=6746"}],"version-history":[{"count":19,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6746\/revisions"}],"predecessor-version":[{"id":7118,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6746\/revisions\/7118"}],"up":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6211"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media\/6780"}],"wp:attachment":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media?parent=6746"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}