{"id":6167,"date":"2023-04-16T15:24:49","date_gmt":"2023-04-16T15:24:49","guid":{"rendered":"https:\/\/er-c.org\/?page_id=6167"},"modified":"2023-10-05T10:57:18","modified_gmt":"2023-10-05T10:57:18","slug":"vitrobot-mark-iv","status":"publish","type":"page","link":"https:\/\/er-c.org\/index.php\/facilities-2\/life-science\/cryo-electron-microscopy-facility\/cryo-em-instrumentation\/cryo-em-sample-preparation\/vitrobot-mark-iv\/","title":{"rendered":"Vitrobot Mark IV"},"content":{"rendered":"\n<h1 class=\"wp-block-heading\">TFS Vitrobot Mark IV  <\/h1>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-1 is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large is-style-default\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"437\" data-id=\"6882\" src=\"https:\/\/er-c.org\/wp-content\/uploads\/2023\/05\/Vitrobot-neu-1024x437.png\" alt=\"\" class=\"wp-image-6882\" srcset=\"https:\/\/er-c.org\/wp-content\/uploads\/2023\/05\/Vitrobot-neu-1024x437.png 1024w, https:\/\/er-c.org\/wp-content\/uploads\/2023\/05\/Vitrobot-neu-300x128.png 300w, https:\/\/er-c.org\/wp-content\/uploads\/2023\/05\/Vitrobot-neu-768x328.png 768w, https:\/\/er-c.org\/wp-content\/uploads\/2023\/05\/Vitrobot-neu-1536x655.png 1536w, https:\/\/er-c.org\/wp-content\/uploads\/2023\/05\/Vitrobot-neu-2048x874.png 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<figcaption class=\"blocks-gallery-caption wp-element-caption\">Left: user (Nuno Bustorff) at the Vitrobot Mark IV device, right: a representative image of the grid prepared by the Vitrobot device (picture credit: Dr. Olivera Korculanin).<\/figcaption><\/figure>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-headline so-widget-sow-headline-default-f8d66e93cdeb\"\n\t\t\t\n\t\t><div class=\"sow-headline-container \">\n\t\t\t\t\t\t<div class=\"decoration\">\n\t\t\t\t\t\t<div class=\"decoration-inside\"><\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div>\n\n\n<h2>Typical applications<\/h2>\n<p>The Vitrobot system is a semi-automated sample preparation instrument that is routinely used in cryo-EM. It removes excess liquid by blotting and is followed by rapid freezing of aqueous sample in liquid ethane giving rise to amorphous vitreous ice.<\/p>\n\n\n<div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-headline so-widget-sow-headline-default-f8d66e93cdeb\"\n\t\t\t\n\t\t><div class=\"sow-headline-container \">\n\t\t\t\t\t\t<div class=\"decoration\">\n\t\t\t\t\t\t<div class=\"decoration-inside\"><\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div>\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<figure class=\"wp-block-table\">\n<h2>Features<\/h2>\n<table style=\"margin: 0px;\">\n<tbody>\n<tr>\n<td style=\"border-style: solid; border-color: #ebebeb;\" scope=\"col\">&#8211; Diverse sample repertoire<\/td>\n<\/tr>\n<tr>\n<td style=\"border-style: solid; border-color: #ebebeb;\" scope=\"col\">&#8211; Easy and flexible user interface<\/td>\n<\/tr>\n<tr>\n<td style=\"border-style: solid; border-color: #ebebeb;\" scope=\"col\">&#8211; Controlled environment<\/td>\n<\/tr>\n<tr>\n<td style=\"border-style: solid; border-color: #ebebeb;\" scope=\"col\">&#8211; High sample throughput<\/td>\n<\/tr>\n<tr>\n<td style=\"border-style: solid; border-color: #ebebeb;\" scope=\"col\">&#8211; Semi-automated grid transfer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Vitrobot system is a semi-automated sample preparation instrument that is routinely used in cryo-EM. It removes excess liquid by blotting and is followed by rapid freezing of aqueous sample in liquid ethane giving rise to amorphous vitreous ice.<\/p>\n","protected":false},"author":2,"featured_media":6172,"parent":6159,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/pagebuilder.php","meta":{"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"class_list":["post-6167","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6167","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/comments?post=6167"}],"version-history":[{"count":12,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6167\/revisions"}],"predecessor-version":[{"id":7478,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6167\/revisions\/7478"}],"up":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6159"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media\/6172"}],"wp:attachment":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media?parent=6167"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}