{"id":6142,"date":"2023-04-16T10:45:24","date_gmt":"2023-04-16T10:45:24","guid":{"rendered":"https:\/\/er-c.org\/?page_id=6142"},"modified":"2023-10-05T11:00:32","modified_gmt":"2023-10-05T11:00:32","slug":"tfs-aquilos-2-cryo-fib","status":"publish","type":"page","link":"https:\/\/er-c.org\/index.php\/facilities-2\/life-science\/cryo-electron-microscopy-facility\/cryo-em-instrumentation\/cryo-em-microscopes\/tfs-aquilos-2-cryo-fib\/","title":{"rendered":"TFS Aquilos 2 Cryo-FIB"},"content":{"rendered":"\n

TFS Aquilos 2 Cryo-FIB <\/h1>\n\n\n\n
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Left: Operater and technician (Dr. Sabrina Berkamp and Pia Sundermeyer) at the Aquilos 2, right: Image of a human RPE1 cells as viewed in Aquilos 2 system under laser, ion and scanning electron microscope beam during different stages of milling (image credit: Dr. Olivera Korculanin)<\/figcaption><\/figure>\n\n\n\n
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Typical applications<\/h2>\n

The Aquilos 2 is a dual beam FIB-SEM platform specifically designed for sectioning of cryo-fixed samples, producing thin lamellae suitable for cryo-electron tomography at an TEM in an automated batch method. Updated with the integrated fluorescence microscopy module – METEOR system and CERES ice shield (Delmic), our Aquilos 2 allows for performing in-situ<\/em> fluorescence microscopy directly on the sample inside the system and reduces transfer steps and associated ice contamination, simultaneously increasing the efficiency of the cryo- correlative light and electron microscopy (CLEM) pipeline. The Aquilos 2 can be used to cut \u201cwindows\u201d in (biological specimen) so cellular processes can be subsequently visualized and later resolved three-dimensionally in their native environment.<\/p>\n\n\n

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Technical specifications<\/h2>\n\n\n\n\n\n\n\n\n\n
Operating voltage<\/td>\nField Emission gun with operation voltage range of 200 V to 30 kV<\/td>\n<\/tr>\n
Resolution of optics<\/td>\n7 nm at 30 kV<\/td>\n<\/tr>\n
Operating conditions<\/td>\nCryo \/ liquid nitrogen conditions<\/td>\n<\/tr>\n
Features<\/td>\nAutomated batch cryo-lamella milling (AutoTEM TFS)
EasyLiftTM <\/sup>Cryo
METEOR Fluorescence module
Ceres Ice shield
Slice and view<\/td>\n<\/tr>\n
Detectors<\/td>\nEverhardt-Thornley external SE detector (ETD)
In-Lens Detection System
METEOR<\/td>\n<\/tr>\n
Stage<\/td>\nFully rotatable (360\u00b0) cryo-stage (<-170\u00b0) with Auto-grid pre-tilted shuttle and a tilt range of -15\u00b0 to +55\u00b0<\/td>\n<\/tr>\n
Software<\/td>\nMaps including image correlation and lamella preparation functions
Auto-TEM for milling automation
Auto Slice & View for 3D volume imaging
Odemis fluorescence software (Delmic)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

The Aquilos 2 is a dual beam FIB-SEM platform specifically designed for sectioning of cryo-fixed samples, producing thin lamellae suitable for cryo-electron tomography at an TEM in an automated batch method. Updated with the integrated fluorescence microscopy module – METEOR system and CERES ice shield (Delmic), our Aquilos 2 allows for performing in-situ fluorescence microscopy directly on the sample inside the system and reduces transfer steps and associated ice contamination, simultaneously increasing the efficiency of the cryo- correlative light and electron microscopy (CLEM) pipeline. The Aquilos 2 can be used to cut \u201cwindows\u201d in (biological specimen) so cellular processes can be subsequently visualized and later resolved three-dimensionally in their native environment.<\/p>\n","protected":false},"author":2,"featured_media":6145,"parent":6072,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/pagebuilder.php","meta":{"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"class_list":["post-6142","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6142","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/comments?post=6142"}],"version-history":[{"count":15,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6142\/revisions"}],"predecessor-version":[{"id":7485,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6142\/revisions\/7485"}],"up":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6072"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media\/6145"}],"wp:attachment":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media?parent=6142"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}