{"id":6133,"date":"2023-04-16T10:17:31","date_gmt":"2023-04-16T10:17:31","guid":{"rendered":"https:\/\/er-c.org\/?page_id=6133"},"modified":"2023-10-05T11:00:04","modified_gmt":"2023-10-05T11:00:04","slug":"tfs-titan-krios-g4","status":"publish","type":"page","link":"https:\/\/er-c.org\/index.php\/facilities-2\/life-science\/cryo-electron-microscopy-facility\/cryo-em-instrumentation\/cryo-em-microscopes\/tfs-titan-krios-g4\/","title":{"rendered":"TFS Titan Krios G4"},"content":{"rendered":"\n

TFS Titan Krios G4 <\/h1>\n\n\n\n
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A and B) Operator and user (Dr. Thomas Heidler and Aikaterini Filopoulou) at the Titan Krios 300 kV C). Micrograph of a vitrified tobacco mosaic virus (TMV) as viewed at 96 kX nominal magnification in Titan Krios 300 kV (picture credit: Dr. Olivera Korculanin)<\/figcaption><\/figure>\n\n\n\n
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Typical applications<\/h2>\n

The Krios G4 (Thermo-Fisher Scientific) is a 300 kV FEG cryo-electron microscope with X-FEG field emitter and a three-lens condenser system. The detector options are a Gatan BioContinuum energy filter mounted K3 direct electron detection camera and a bottom mounted Falcon 4(i) detector (FEI). Combined with fringe-free imaging (FFI) and aberration-free image shift (AFIS) in EPU, high-throughput imaging rates can be achieved. The microscope can be tuned set up for single-particle imaging, STEM and tomography experiments. Additionally, the Titan Krios is equipped with a Panther detector system (4 segments and annular dark-field detectors) that can be used for iDPC and HAADF-STEM imaging. The acquisition rate for a single particle sample on the Titan Krios is ~250-700 acquisitions\/hour and we have achieved a maximal resolution of 1.7 \u00c5 with an apo-ferritin test sample.<\/p>\n\n\n

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Technical specifications<\/h2>\n\n\n\n\n\n\n\n\n\n\n
Operating voltage<\/td>\n80 kV – 300 kV
Alignments for 120, 200 and 300kV<\/td>\n<\/tr>\n
TEM magnification range<\/td>\nUp to 195,000X with Falcon IV (0.38 \u00c5\/pixel)
165,000X with K3 detector (0.52 \u00c5\/pixel and 0.26 \u00c5\/pixel in super res mode)<\/td>\n<\/tr>\n
Ion source<\/td>\nX-FEG<\/td>\n<\/tr>\n
Operating conditions<\/td>\nCryo \/ liquid nitrogen conditions<\/td>\n<\/tr>\n
Detector<\/td>\nCeta 4kx4k 16M camera
Gatan K3 camera (including energy filter BioContinuum GIF)
Falcon 4 camera
Panther segmented STEM detector
Fischione annular dark-field STEM detector<\/td>\n<\/tr>\n
Configuration<\/td>\nAutoloader with up to 12 grids
Automated data acquisition at cryo-conditions
Volta phase plate<\/td>\n<\/tr>\n
Stage<\/td>\nComputerized 4-axes specimen stage with \u00b170\u00b0 alpha tilt
Dual tilt cryostage<\/td>\n<\/tr>\n
Software<\/td>\nTIA, EPU, Velox, SerialEM, Maps, Tomo<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

The Krios G4 (Thermo-Fisher Scientific) is a 300 kV FEG cryo-electron microscope with X-FEG field emitter and a three-lens condenser system. The detector options are a Gatan BioContinuum energy filter mounted K3 direct electron detection camera and a bottom mounted Falcon 4(i) detector (FEI). Combined with fringe-free imaging (FFI) and aberration-free image shift (AFIS) in EPU, high-throughput imaging rates can be achieved. The microscope can be tuned set up for single-particle imaging, STEM and tomography experiments. Additionally, the Titan Krios is equipped with a Panther detector system (4 segments and annular dark-field detectors) that can be used for iDPC and HAADF-STEM imaging. The acquisition rate for a single particle sample on the Titan Krios is ~250-700 acquisitions\/hour and we have achieved a maximal resolution of 1.7 \u00c5 with an apo-ferritin test sample.<\/p>\n","protected":false},"author":2,"featured_media":6140,"parent":6072,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/pagebuilder.php","meta":{"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"class_list":["post-6133","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6133","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/comments?post=6133"}],"version-history":[{"count":12,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6133\/revisions"}],"predecessor-version":[{"id":7484,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6133\/revisions\/7484"}],"up":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6072"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media\/6140"}],"wp:attachment":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media?parent=6133"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}