{"id":6091,"date":"2023-04-16T08:52:43","date_gmt":"2023-04-16T08:52:43","guid":{"rendered":"https:\/\/er-c.org\/?page_id=6091"},"modified":"2023-10-05T10:59:03","modified_gmt":"2023-10-05T10:59:03","slug":"tfs-talos-l120c-em","status":"publish","type":"page","link":"https:\/\/er-c.org\/index.php\/facilities-2\/life-science\/cryo-electron-microscopy-facility\/cryo-em-instrumentation\/cryo-em-microscopes\/tfs-talos-l120c-em\/","title":{"rendered":"TFS Talos L120C EM"},"content":{"rendered":"\n

TFS Talos L120C EM <\/h1>\n\n\n\n
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Left: Operator (Dr. Iris von der Hocht) loading a sample at room temperature into the Talos 120 kV EM, right: Micrograph of the tobacco mosaic virus (TMV) particles as captured by the Talos 120 kV<\/figcaption><\/figure>\n\n\n\n
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Typical applications<\/h2>\n

The Talos L120C Electron microscope is a robust tool routinely used for initial screening of a biological sample’s purity and homogeneity. The main application at the ER-C is negative staining – a heavy metal embedding of purified proteins at room temperature (see image). In addition, the evaluation of sample quality can also be performed at cryogenic temperatures.<\/p>\n\n\n

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Technical specifications<\/h2>\n\n\n\n\n\n\n\n\n
Operating voltage<\/td>\n120 kV<\/td>\n<\/tr>\n
Ion source<\/td>\nLaB6 filament<\/td>\n<\/tr>\n
Operating conditions<\/td>\nRT and cryo \/ liquid nitrogen conditions<\/td>\n<\/tr>\n
Detector<\/td>\nFast 4k \u00d7 4k Ceta 16M camera CMOS (TFS)<\/td>\n<\/tr>\n
Holders<\/td>\n677 Multispecimen holder (Gatan)
626 Single tilt cryo-transfer holder (Gatan)
Single tilt TFS<\/td>\n<\/tr>\n
Software<\/td>\nEPU,Velox, TIA<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n
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The Talos L120C Electron microscope is a robust tool routinely used for initial screening of a biological sample’s purity and homogeneity. The main application at the ER-C is negative staining – a heavy metal embedding of purified proteins at room temperature (see image). In addition, the evaluation of sample quality can also be performed at cryogenic temperatures.<\/p>\n","protected":false},"author":2,"featured_media":6128,"parent":6072,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/pagebuilder.php","meta":{"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"class_list":["post-6091","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6091","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/comments?post=6091"}],"version-history":[{"count":25,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6091\/revisions"}],"predecessor-version":[{"id":7482,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6091\/revisions\/7482"}],"up":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6072"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media\/6128"}],"wp:attachment":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media?parent=6091"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}