{"id":10653,"date":"2025-06-25T19:29:47","date_gmt":"2025-06-25T19:29:47","guid":{"rendered":"https:\/\/er-c.org\/?page_id=10653"},"modified":"2025-06-25T19:37:21","modified_gmt":"2025-06-25T19:37:21","slug":"tfs-titan-arctis-cryo-plasma-fib","status":"publish","type":"page","link":"https:\/\/er-c.org\/index.php\/facilities-2\/life-science\/cryo-electron-microscopy-facility\/cryo-em-instrumentation\/cryo-em-microscopes\/tfs-titan-arctis-cryo-plasma-fib\/","title":{"rendered":"TFS Arctis Cryo-Plasma FIB"},"content":{"rendered":"\n

TFS Arctis Cryo-Plasma FIB<\/h1>\n\n\n\n
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Left: Arctis cryo-plasma focussed ion beam (PFIB) scanning electron microscope (SEM); Right: SEM images of lamellae milled by xenon (top, target thickness of 150, 175 and 200 nm) and argon (bottom, arget thickness of 200 nm) PFIB, for cryo-frozen baker\u2019s yeast cells using Arctis WebUI conventional lamella workflow\u2019s optimized templates.<\/figcaption><\/figure>\n\n\n\n
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Typical applications<\/h2>\n

The ThermoFisher Scientific Arctis cryo-plasma focussed ion beam (cryo-PFIB) is a state-of-the-art dual-beam FIB-scanning electron microscope (SEM) specifically engineered for preparing frozen hydrated cellular samples for cryo-electron tomography (cryo-ET). It is ideally suited for producing thin lamellae for an automated cryo-ET workflow, due to its high-precision, reproducibility and high-throughput capabilities. This system leverages an advanced plasma ion source, offering versatile milling with xenon, argon, or oxygen ions, ensuring rapid and precise material removal without gallium contamination. A key feature is its integrated fluorescence microscopy (iFLM) correlative system, allowing in-situ fluorescence imaging directly on the cryo-sample, reducing transfer steps and minimizing ice contamination. Together, these steps significantly boost the efficiency of cryo-correlative light and electron microscopy (CLEM) pipeline. The Arctis cryo-PFIB includes an  autoloader system that can handle up to 12 cryogenically frozen grids, minimizing manual intervention and ensuring consistent lamella alignment.<\/p>\n\n\n

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Technical specifications<\/h2>\n\n\n\n\n\n\n\n\n\n
Electron Optics<\/strong><\/td>\n\n

Source: High-stability Schottky field emission gun;
Beam Current: 1.5 pA to 400 nA;
Accelerating Voltage: 0.2 kV to 30 kV;
Detectors: In-lens detection system: T1 (BSE) and T2 (SE); In-chamber ETD (SE)
Resolution (T2): <2.6 nm at 2 kV<\/p>\n<\/td>\n<\/tr>\n

Ion Optics<\/strong><\/td>\n\n

Source: PFIB with inductively coupled plasma (ICP) source;
Ion Species: Xenon, Argon and Oxygen;
Beam Current: 1.5 pA to 2.5 \u00b5A;
Accelerating Voltage: 0.5 kV to 30 kV;
Resolution (Xe+ beam): <20 nm at 30 kV<\/p>\n<\/td>\n<\/tr>\n

Integrated Fluorescence Microscopy (iFLM)<\/strong><\/td>\n\n

Type: Wide-field fluorescence light microscope;
Objective: 100x Zeiss Epiplan Neofluar NA 0.75;
Working Distance: 4 mm;
Modes: Fluorescence and Reflection;
Filters: 4-channel Quadband (DAPI, FITC, TRITC and Cy5 optimized);
Camera: Basler a2A4504-18umPRO (20.2 MP resolution);
LED Source: CoolLED, 4 channels (365 nm\/450 nm\/550 nm\/ 635 nm)<\/p>\n<\/td>\n<\/tr>\n

Vacuum System<\/strong><\/td>\n\n

Nitrogen-cooled Autoloader and CompuStage with dedicated cryo-box for shielding against water condensation;
Vacuum chamber pressure: <5 \u00d7 10-5<\/sup> Pa (at cryo-conditions)<\/p>\n<\/td>\n<\/tr>\n

Sample Stage<\/strong><\/td>\n\n

CompuStage; computerized 4-axis cryo-stage with \u00b190-degree alpha tilt;
Eucentric point: 10 mm from electron column pole piece<\/p>\n<\/td>\n<\/tr>\n

Autoloader Capacity<\/strong><\/td>\n\n

Up to 12 cryogenically frozen grid carriers (compatible with TomoGrids, AutoGrids, FIB-AutoGrids)<\/p>\n<\/td>\n<\/tr>\n

Softwares<\/strong><\/td>\n\n

Web-Based User Interface (WebUI): remote operation;
xT Microscope Control: works in conjunction with the WebUI;
AutoTEM Cryo2.4;
Maps3.25<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

The Krios G4 (Thermo-Fisher Scientific) is a 300 kV FEG cryo-electron microscope with X-FEG field emitter and a three-lens condenser system. The detector options are a Gatan BioContinuum energy filter mounted K3 direct electron detection camera and a bottom mounted Falcon 4(i) detector (FEI). Combined with fringe-free imaging (FFI) and aberration-free image shift (AFIS) in EPU, high-throughput imaging rates can be achieved. The microscope can be tuned set up for single-particle imaging, STEM and tomography experiments. Additionally, the Titan Krios is equipped with a Panther detector system (4 segments and annular dark-field detectors) that can be used for iDPC and HAADF-STEM imaging. The acquisition rate for a single particle sample on the Titan Krios is ~250-700 acquisitions\/hour and we have achieved a maximal resolution of 1.7 \u00c5 with an apo-ferritin test sample.<\/p>\n","protected":false},"author":2,"featured_media":6140,"parent":6072,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/pagebuilder.php","meta":{"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"class_list":["post-10653","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/10653","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/comments?post=10653"}],"version-history":[{"count":5,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/10653\/revisions"}],"predecessor-version":[{"id":10660,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/10653\/revisions\/10660"}],"up":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/pages\/6072"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media\/6140"}],"wp:attachment":[{"href":"https:\/\/er-c.org\/index.php\/wp-json\/wp\/v2\/media?parent=10653"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}